Efficacy of an ultrasound technique in the recovery of spermatozoa during the investigation of sexual offenses
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Lezcano Martínez, Ana Nataly | 2019
Objective: To evaluate the efficiency for the recovery of spermatozoa using the standard technique
and a technique based on ultrasound.
Materials and methods: An experimental study was carried out to compare two techniques for
spermatozoa recovery; (i) the standard technique, based on elution of the evidence in distilled
water with a buffer and, (ii) a modified technique that included concentration by centrifugation
and ultrasound. The efficiency was based on manual counting of spermatozoa using dilutions
1:1 to 1:500.
Results: The modified technique substantially improved the recovery of spermatozoa. While
the standard technique recovered 3.1%, the modified one resulted in a recovery of 17.5% using
the 1:10 dilution, which represents a six-fold increase. The modified technique was the only
one able to recover spermatozoa in dilutions higher than 1:250.
Conclusion: The implementation of the ultrasonic technique improves the recovery of
spermatozoa.
LEER